Accurate dosage of antibiotics in extended boar semen

Key features of the ADA protocol

• The ejaculate is diluted in a two-step process. Antibiotic-free extender is used for the main dilution step. An antibiotic concentrate is added separately.
• The calculated end-volume of the finally diluted ejaculate defines the required volume of the antibiotic concentrate as well as the volume of the antibiotic free main extender.
• The same amount of antibiotics is added per sperm cell, independent of the dilution ratio.

Benefits of the ADA concept

• Accurate dosage of antibiotics in every ejaculate secures semen quality in each dose
• The fully automated protocol is easy to integrate into existing operating processes
• Main extender contains no antibiotics, therefore less antibiotics are discarded, reducing the negative impact on the environment
• Dust free handling of antibiotics protects lab personnel and minimizes the risk of building up antibiotic resistances

 
How the ADA protocol works

• Automated calculation of the end volume of diluted ejaculate based on sperm analysis:
  To facilitate the ADA process in the boar semen laboratory, the complete calculation and dilution process is performed fully automated by Prism10 laboratory software and the SmartDispenser 3.0.
• Add AB concentrate: 
  The innovative water-soluble foil of the ADA-pouch enables non-contact and dust-free preparation of the liquid antibiotic concentrate. When dropped into water the ADA-pouch dissolves completely.
• Add AB-free main extender

 Watch our video for more information on how ADA can optimize your lab process!